描述

pETM11 vector carries two His•Tag and MAD sequences. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer.

详细说明

出品公司：EMBL Protein Expression and Purification Facility

载体类型：原核表达载体

启动子：T7

关键功能：IPTG诱导表达

原核抗性：Kan

检测标签：His•Tag