产品简介：

The pET-28a-c(+) vectors carry an N-terminal His•Tag®/ thrombin/ T7•Tag® configuration plus an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymeraseis shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer.

产品参数：

质粒类型：大肠杆菌表达载体

出品公司：Novagen

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：5368 bp

5' 测序引物：T7

3' 测序引物：T7-ter

载体标签：N-His, N-Thrombin, N-T7, C-His

载体抗性：Kanamycin (卡那霉素)

备注：载体带有N端凝血酶蛋白酶切位点; pET28a, b, c的差异主要是在多克隆位点。