描述

The Glutathione S-transferase (GST) Gene Fusion System from GE Healthcare is a versatile system for the expression, purification, and detection of GST-tagged proteins produced in E. coli. GST occurs naturally as an Mr 26 000 protein, which can be expressed in E. coli with full enzymatic activity. The pGEX plasmid vectors are designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with S. japonicum GST. Recombinant proteins are easily purified from, for example E. coli cell lysates by affinity chromatography using Glutathione Sepharose media, either with prepacked columns or by batch purification. Cleavage of the target protein from GST is achieved using a site-specific protease, which possesses a recognition sequence located immediately upstream from the multiple cloning site on the pGEX vectors.

详细说明

出品公司：GE Healthcare

载体类型：原核表达载体

启动子：Tac

关键功能：IPTG诱导表达

原核抗性：Amp

检测标签：GST•Tag