描述:

pMXB10 is a control plasmid for the IMPACT Kit. This plasmid carries the E. coli malE gene, encoding the maltose binding protein (MBP, 42 kDa), fused in-frame to the coding region of the Mxe gyrase A intein-chitin binding domain (28 kDa) from pTXB1. pMXB10 can be used to test plasmid transformation, cell culture, induction and purification procedures. After induction with 0.3 mM IPTG at 30°C for 3 hours (or 15°C for 12-16 hours), 100 ml of cells should yield 2-3 mg of a 70 kDa fusion protein. After chitin column purification and cleavage, approximately 1.5-2.0 mg of MBP is usually obtained. This double stranded vector is 7813 bp in length.

详细说明:

出品公司：NEB

载体类型：原核表达载体

  启动子：T7

关键功能：实验对照

原核抗性：Amp

检测标签：无