描述：

pMAL-p5G is an E. coli plasmid cloning vector designed for recombinant protein expression and purification using the pMAL Protein Fusion and Purification System. It contains the pMB1 origin of replication from pBR322 and is maintained at a similar copy number to pBR322. The multiple cloning site (MCS) is positioned to allow translational fusion of the E. coli maltose binding protein (MBP, encoded by the malE gene) to the N-terminus of the cloned target protein. The pMAL-p5 and -c5 series of vectors differs from the -p4 and -c4 series in that they contain a universal multiple cloning site (MCS) that is compatible with other NEB expression systems and is followed by stop codons in all three reading frames. In addition, lacZα and the M13 origin have been removed. pMAL-p5G is identical to pMAL-p5X except it replaces the Factor Xa protease cleavage site with Genenase I cleavage sites.

详细说明：

出品公司：NEB

载体类型：原核表达载体

  启动子：P_tac

关键功能：融合表达MBP蛋白

原核抗性：Amp