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pET-35b(+) 
   Cat.No QYV0650    
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20 reactions 1200.00 1200.00
1
   
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20 reactions
Documentation and quality management
COA Datasheet
Basic product information:

产品简介:

Cellulose binding domains(CBDs) occur in nature as discrete regions of cellulases and cellulosome scaffolding proteins. Cellulose binding domains provide a new class of affinity tags with many hiahly desirable attributes, including:

1. a readily available,low cost affinity matrix (cellulose) with inherent low non-specific protein binding characteristics.

2.high affinity binding that facilitates rapid purification from dilute and/or complex samples.

3.efficient release under nondenaturing conditions,and.

4.effective immobilization of CBD fusion proteins to cellulose-based matrices without the need for covalent crosslinking.

Then pET-35b(+) vector encodes the CBD clos domain and direct expression to the E.coli cytoplasm.

 

产品参数:

质粒类型:原核表达载体

出品公司:Novagen

启动子:T7-lac

克隆方法:多克隆位点,限制性内切酶

5' 测序引物及序列:T7

3' 测序引物及序列:5'-TAATACGACTCACTATAGGG-3'

载体标签:N-CBDclos,C-His

载体抗性:Kanamycin(卡那霉素)

 

Other technical information

For more information, please download and refer to the product manual。

Please send your questions to us,thank you for your support!