载体介绍：

pBudCE4.1 is a 4.6 kb vector designed for simultaneous expression of two genes in mammalian cell lines. The vector contains the human cytomegalovirus (CMV) immediate-early promoter and the human elongation factor 1α-subunit (EF-1α) promoter for high-level, constitutive, independent expression of two recombinant proteins. Features of the vector allow detection and purification of expressed proteins. High-level stable and transient expression studies can be carried out in most mammalian cell types. In addition to the two promoters, the vector contains the following elements:
（1）C-terminal peptides encoding the myc (c-myc) epitope or the V5 epitope and a polyhistidine (6xHis) metal-binding tag for detection and purification of recombinant proteins.
（2）Zeocin™ resistance gene for selection in E. coli and creation of stable, mammalian cell lines (Mulsant et al., 1988). 
（3）SV40 origin for episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g., COS7). 

载体特点：

pBudCE4.1是一个双表达框哺乳动物表达载体，含有两个强启动子（CMV启动子和EF-1α启动子），两个启动子各调控一个表达框，互不干扰，从而可以实现同时表达两个基因、且两者表达互不影响的目的。如果在EF-1α启动子后面插入EGFP或者其它荧光基因，CMV启动子后插入外源基因，则可以做到既有荧光蛋白来做转染指示，且荧光基因表达和外源基因表达又不会互相干扰。

产品参数：

质粒类型：哺乳动物表达载体(双启动子)

启动子：EF-1α, CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：4595 bp

5' 测序引物及序列：CMVPro-F:5'd[TAATACGACTCACTATAGGG]3'

EF1aPro-F:5'd[TCAAGCCTCAGACAGTGGTTC]3'

载体标签：6xHis, c-Myc, V5

载体抗性：Zeocin (博莱霉素)

筛选标记：Zeocin (博莱霉素)

备注：能够利用该载体同时表达两种基因