English
   中文版
E-mail: qualityard@163.com    Tel: 400-812-0026
Home 细胞与载体资源 哺乳动物细胞表达系统
<
>
pEF1/myc-His B 
   Cat.No QYV0510    
Size
List Price
Promotion Price
Stock
Purchase
20 reactions 1200.00 1200.00
5
   
图片描述:
20 reactions
Documentation and quality management
COA Datasheet
Basic product information:

载体介绍:

pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

  1. Human elongation factor 1?-subunit (hEF-1α) promoter for high-level expression across a broad range of species and cell types (Goldman et al., 1996; Mizushima and Nagata, 1990).

  2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc (c-myc) epitope and a polyhistidine metal-binding peptide.

  3. Neomycin resistance gene for selection of stable cell lines.

  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g., COS7).

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

产品参数:

质粒类型:哺乳动物表达载体

表达水平:高拷贝

启动子:EF-1a

载体大小:6169 bp

5' 测序引物及序列:T7 Fwd:5'd[TAATACGACTCACTATAGGG]3'

载体标签:6X His, myc

载体抗性:Ampicillin (氨苄青霉素)

筛选标记:Neomycin

 

Other technical information

For more information, please download and refer to the product manual。

Please send your questions to us,thank you for your support!