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  •  Western Blot analysis of 3T3 cells using Phospho-GRF-1 (Y1105) Polyclonal Antibody
  •  Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  •  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRF-1 (Phospho-Tyr1105) Antibody. The picture on the right is blocked with the phospho peptide.
  •  Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using GRF-1 (Phospho-Tyr1105) Antibody. The lane on the right is blocked with the phospho peptide.
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GRF-1 (phospho Tyr1105) Polyclonal Antibody 
   Cat.No QYA04184    
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100ug/100ul 2250.00 1350.00
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100ug/100ul
Documentation and quality management
COA Datasheet
Basic product information:

Catalog No.

QYA04184

Size.

100ug

Gene Name.

ARHGAP35

Protein Name.

Rho GTPase-activating protein 35

Source.

Rabbit

Immunogen.

Synthesized phospho-peptide around the phosphorylation site of human GRF-1 (phospho Tyr1105)

Purification.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity.

Phospho-GRF-1 (Y1105) Polyclonal Antibody detects endogenous levels of GRF-1 protein only when phosphorylated at Y1105.

Formulation.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration.

1 mg/ml

Storage / Stability.

-20°C/1 year

Human Gene ID.

2909

Human Swiss-Prot No.

Q9NRY4

Mouse Swiss-Prot No.

Q91YM2

Rat Swiss-Prot No.

P81128

Reactivity.

Human, Mouse, Rat

Applications.

WB,IHC-p,ELISA

Predicted band (Da)

172229

Observed band (KDa)

160

Dilution.

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Other name.

ARHGAP35; GRF1; GRLF1; KIAA1722; Rho GTPase-activating protein 35; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-A

Other technical information

For more information, please download and refer to the product manual。

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