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  •  Western Blot analysis of COLO cells using Phospho-GRB10 (Y67) Polyclonal Antibody
  •  Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  •  Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using GRB10 (Phospho-Tyr67) Antibody
  •  Immunofluorescence analysis of HepG2 cells, using GRB10 (Phospho-Tyr67) Antibody. The picture on the right is blocked with the phospho peptide.
  •  Western blot analysis of lysates from NIH/3T3 cells treated with Insulin 0.01U/ml 15', using GRB10 (Phospho-Tyr67) Antibody. The lane on the right is blocked with the phospho peptide.
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GRB10 (phospho Tyr67) Polyclonal Antibody 
   Cat.No QYA04176    
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100ug/100ul 2250.00 1350.00
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100ug/100ul
Documentation and quality management
COA Datasheet
Basic product information:

Catalog No.

QYA04176

Size.

100ug

Gene Name.

GRB10

Protein Name.

Growth factor receptor-bound protein 10

Source.

Rabbit

Immunogen.

Synthesized phospho-peptide around the phosphorylation site of human GRB10 (phospho Tyr67)

Purification.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity.

Phospho-GRB10 (Y67) Polyclonal Antibody detects endogenous levels of GRB10 protein only when phosphorylated at Y67.

Formulation.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration.

1 mg/ml

Storage / Stability.

-20°C/1 year

Human Gene ID.

2887

Human Swiss-Prot No.

Q13322

Mouse Swiss-Prot No.

Q60760

Rat Swiss-Prot No.

 

Reactivity.

Human

Applications.

WB,IHC-p,IF,ELISA

Predicted band (Da)

67231

Observed band (KDa)

67

Dilution.

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Other name.

GRB10; GRBIR; KIAA0207; Growth factor receptor-bound protein 10; GRB10 adapter protein; Insulin receptor-binding protein Grb-IR

Other technical information

For more information, please download and refer to the product manual。

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