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  •  Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using FKHRL1 (Phospho-Ser253) Antibody
  •  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FKHRL1 (Phospho-Ser253) Antibody. The picture on the right is blocked with the phospho peptide.
  •  Western blot analysis of lysates from LOVO cells treated with serum 20% 30', using FKHRL1 (Phospho-Ser253) Antibody. The lane on the right is blocked with the phospho peptide.
  •  Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  •  Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  •  Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  •  Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  •  Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  •  Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  •  Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  •  Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  •  Western Blot analysis of HELA cells using Phospho-FoxO3A (S253) Polyclonal Antibody diluted at 1:2000
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FoxO3A (phospho Ser253) Polyclonal Antibody 
   Cat.No QYA03692    
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100ug/100ul 2250.00 1350.00
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100ug/100ul
Documentation and quality management
COA Datasheet
Basic product information:

Catalog No.

QYA03692

Size.

100ug

Gene Name.

FOXO3

Protein Name.

Forkhead box protein O3

Source.

Rabbit

Immunogen.

Synthesized phospho-peptide around the phosphorylation site of human FoxO3A (phospho Ser253)

Purification.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity.

Phospho-FoxO3A (S253) Polyclonal Antibody detects endogenous levels of FoxO3A protein only when phosphorylated at S253.

Formulation.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration.

1 mg/ml

Storage / Stability.

-20°C/1 year

Human Gene ID.

2309

Human Swiss-Prot No.

O43524

Mouse Swiss-Prot No.

Q9WVH4

Rat Swiss-Prot No.

 

Reactivity.

Human, Mouse, Rat

Applications.

WB,IHC-p,IF,ELISA

Predicted band (Da)

71277

Observed band (KDa)

97

Dilution.

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Other name.

 
Other technical information

For more information, please download and refer to the product manual。

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