Size | List Price |
Promotion Price | Stock |
Purchase |
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20 reactions | 1200.00 | 1200.00 | 2 |
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20 reactions |
pCMV-AD 载体
质粒类型: 哺乳动物双杂交系统
高拷贝/低拷贝: 高拷贝
启动子: CMV
载体大小: 4.2kb
载体抗性: Ampicillin (氨苄青霉素)
载体描述
The pCMV-BD and pCMV-AD vectors are designed for the construction and expression of gene fusions with the GAL4 DNA binding domain and NF-κB transcriptional activation domain, respectively. The pCMV-BD vector contains DNA encoding amino acids 1–147 of the GAL4 gene (DNA binding domain)2 and unique 3′ cloning sites. It is used for the construction of bait plasmids containing a DNA insert encoding a bait protein. The pCMV-AD vector contains DNA encoding the nuclear localization sequence (NLS) from SV40 large T-antigen (PKKKRKV), amino acids 364–550 of the mouse NF-κB gene (transcriptional activation domain)3 and unique 3′ cloning sites. It is used for constructing a target plasmid containing a DNA insert encoding the interacting proteins. Both pCMV-BD and pCMV-AD contain the pUC origin for replication in E. coli. The pCMV-BD vector contains the kanamycin-resistance gene and the pCMV-AD vector contains the ampicillin-resistance gene. The constitutively-active cytomegalovirus (CMV) promoter governs the expression of the bait and target proteins in the pCMV-BD and pCMV-AD vectors. The SV40 poly (A) provide the signals necessary for transcriptional termination and polyadenylation of the bait and target genes in mammalian cells.
Product Name | Thumbnail |
---|---|
pACT | |
pBIND | |
pACT-MyoD | |
pBIND-Id | |
pG5 luc | |
pCMV-BD | |
pBD-p53 | |
pBD-NF−κB | |
pAD-SV40T | |
pAD-TRAF | |
pFR-luc | |
pGBKT7 | |
pGADT7 | |
pCL1 | |
pGBKT7-53 |
For more information, please download and refer to the product manual。