Size | List Price |
Promotion Price | Stock |
Purchase |
---|---|---|---|---|
20 reactions | 1500.00 | 1500.00 | 3 |
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图片描述: | ||||
20 reactions |
载体介绍:
wo additional shuttle vectors, pShuttle-IRES-hrGFP-1 (Catalog #240081) and pShuttle-IRES-hrGFP-2 (Catalog #240082) are available separately. Both of these vectors contain the CMV promoter and a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES, which directs translation of a humanized recombinant green fluorescent protein (hrGFP) from a novel marine organism as a second open reading frame. This design allows the expression of the gene of interest (up to 5.2 kb) to be monitored at the single-cell level due to expression of the hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG® epitope (pShuttle-IRES-hrGFP-1) or the HA epitope (pShuttle-IRES-hrGFP-2).
产品参数:
质粒类型:腺病毒系统
高拷贝/低拷贝:低拷贝
启动子: CMV
克隆方法:多克隆位点,限制性内切酶
载体大小:8.9 kb
5' 测序引物及序列:pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC
3' 测序引物及序列:pShuttle-IRES-hrGFP-1-R: ATGCAGTCGTCGAGGAATTG
载体抗性:Kanamycin (卡那霉素)
For more information, please download and refer to the product manual。