Size | List Price |
Promotion Price | Stock |
Purchase |
---|---|---|---|---|
20 reactions | 1800.00 | 1800.00 | 3 |
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20 reactions |
产品参数
质粒类型:细胞重编程载体(iPS)
启动子:CMV
克隆方法:多克隆位点,限制性内切酶
载体大小:13kb
载体标签:GFP
载体抗性:Ampicillin (氨苄青霉素)
载体介绍
pLentG-KOSM is a simple lentiviral vector in which defined factors are in-frame fused into a single open reading frame (ORF) via self-cleaving 2A peptides and are controlled by a CMV promoter. The mouse transcription factor ORF is followed by IRES-GFP as a reporter for viral transduction. This polycistronic expression system can efficiently reprogram mouse or human somatic cells into iPS cells; for example, in infected MEF (GFP+), the reprogramming efficiency is around 1%, which is at least 10-fold higher than the reprogramming method using a pool of viruses in which each virus expresses a different stem cell factor gene. Notably, most of the iPS cells generated using the pLentG-KOSM system contain only a single copy of the viral vector (Ref. 1).
For more information, please download and refer to the product manual。