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Home 细胞与载体资源 果蝇细胞表达系统
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pMT-Bip-V5-HisA 
   Cat.No QYV0623    
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20 reactions 1800.00 1800.00
3
   
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20 reactions
Documentation and quality management
COA Datasheet
Basic product information:

产品简介:

The DES®-Inducible/Secreted Kit provides the vector pMT/BiP/V5-His for inducible, secreted expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector offers the inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride. The N-terminal signal sequence from the insect BiP gene is provided to direct the recombinant fusion protein through the secretory pathway of S2 cells into the culture medium. The pMT/BiP/V5-His vector offers the following additional features:

  • Small size (3.6 kb) to improve DNA yields and increase subcloning efficiency

  • C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody

  • C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the BiP signal sequence.

 

产品参数:

质粒类型:昆虫细胞表达载体

出品公司:Invitrogen

启动子:MT

载体大小:3642 bp

载体标签:V5 Epitope Tag, His Tag (6x)

载体抗性:Ampicillin (氨苄青霉素)

 

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pAc5.1 V5-His B pAc5.1 V5-His B
pCoHygro pCoHygro
pCoBlast pCoBlast
Other technical information

For more information, please download and refer to the product manual。

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