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Home 细胞与载体资源 果蝇细胞表达系统
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pAc5.1 V5-His B 
   Cat.No QYV0622    
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20 reactions 1500.00 1500.00
3
   
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20 reactions
Documentation and quality management
COA Datasheet
Basic product information:

产品简介:

The DES®-Constitutive Kit provides the vector pAc5.1/V5-His for high-level constitutive expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. The vector offers the strong, constitutive promoter from the Drosophila actin 5C gene. The pAc5.1/V5-His vector also offers the following features:
Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency.
C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody.
C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin.

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.

 

产品参数:

质粒类型:果蝇细胞表达系统

出品公司:Invitrogen

启动子:P-AC5 (Actin 5C)

克隆方法:多克隆位点,限制性内切酶

载体大小:5379bp

5' 测序引物及序列:AC5:5'd[ACACAAAGCCGCTCCATCAG]3'

载体标签:V5 (Cterm), His (Cterm)

载体抗性:Ampicillin (氨苄青霉素)

 

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pMT-Bip-V5-HisA pMT-Bip-V5-HisA
pCoHygro pCoHygro
pCoBlast pCoBlast
Other technical information

For more information, please download and refer to the product manual。

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