Size | List Price |
Promotion Price | Stock |
Purchase |
---|---|---|---|---|
20 reactions | 1500.00 | 1500.00 | 5 |
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图片描述: | ||||
20 reactions |
产品简介:
pOG44 is a 5.8 kb Flp recombinase expression vector designed for use with the Flp-In. System (Catalog nos. K6010-01 and K6010-02) available from Invitrogen. When cotransfected with the pcDNA5/FRT plasmid into a Flp-In. mammalian host cell line, the Flp recombinase expressed from pOG44 mediates integration of the pcDNA5/FRT vector containing the gene of interest into the genome via Flp Recombination Target (FRT) sites.
The vector contains the following elements:
·The human cytomegalovirus (CMV) immediate-early enhancer/promoter for highlevel constitutive expression of the Flp recombinase in a wide range of mammalian cells (Andersson et al., 1989; Boshart et al., 1985; Nelson et al., 1987)
·Synthetic intron to enhance expression of the FLP gene (Huang and Gorman, 1990; O’Gorman et al., 1991)
·FLP gene encoding the Flp recombinase (Buchholz et al., 1996) to mediate integration of the pcDNA5/FRT expression plasmid into the genome
产品参数:
质粒类型:哺乳细胞表达载体
出品公司:Invitrogen
启动子:CMV
克隆方法:多克隆位点,限制性内切酶
载体大小:5785bp
载体抗性:Ampicillin (氨苄青霉素)
Product Name | Thumbnail |
---|---|
pFRT/lacZeo | |
pcDNA5/FRT | |
pcDNA5/FRT/CAT |
For more information, please download and refer to the product manual。