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Home 细胞与载体资源 昆虫细胞表达系统
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pAcGP67A 
   Cat.No QYV0582    
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20 reactions 1350.00 1350.00
2
   
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20 reactions
Documentation and quality management
COA Datasheet
Basic product information:

产品简介:

The acidic glycoprotein gp67 (syn.: gp64) is the most abundant envelope surface glycoprotein of the Autographa californica nuclear polyhedrosis virus (AcNPV baculovirus), and is essential for the entry of baculovirus particles into susceptible insect cells. Since large amounts of this protein are secreted and anchored to the virus peplomer, its gene contains one of the most effective baculovirus-encoded signal sequences for protein secretion. Therefore, we have constructed baculovirus transfer vectors that contain the gp67 signal sequence in front of a multiple cloning site. A gene of choice can be inserted in one of these cloning sites and the protein of interest will be expressed as a gp67 signal peptide fusion protein under the control of the strong baculovirus polyhedrin promoter. This strategy allows the forced secretion of otherwise non-secreted recombinant proteins which may be easily purified when serum-free insect culture medium, BD BaculoGold Max-XP Insect Cell Medium is used. The transfer vector(s) should be preferentially used in conjunction with BD BaculoGold DNA .

 

产品参数:

质粒类型昆虫杆状病毒表达载体

出品公司:BD Biosciences

启动子polyhedrin

载体大小9761 bp

载体抗性Ampicillin

备注含有GP67分泌信号肽
 

 

Other technical information

For more information, please download and refer to the product manual。

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