载体简介：

The pSingle-tTS-shRNA vector expresses the tetracyline-controlled transcriptional suppressor (tTS), which controls expression of an shRNA sequence inserted into the shRNA cloning site. The tTS protein is a fusion of the Tet repressor protein (TetR) and the KRAB-AB silencing domain of the Kid-1 protein (SDKid-1 ), a powerful transcriptional suppressor (1, 2). In the absence of the inducer doxycycline (Dox, a tetracycline derivative), tTS binds to the tetO sequences in the modified Tet-responsive Pol III hybrid promoter (P Tight/U6 ) and blocks expression of the shRNA. As Dox is added to the culture medium, tTS dissociates from P Tight/U6 to allow Pol IIImediated transcription of the shRNA, resulting in suppression of the target gene in a highly dose-dependent manner. pSingle-tTS-shRNA also contains a ColE1 origin of replication and an ampicillin resistance gene (Amp r ) for propagation and selection in bacteria, as well as a neomycin resistance gene (Neo r ) for selection of stable transformants in mammalian cells.

产品参数：

出品公司：Genscript

质粒类型:RNAi载体

克隆方法:多克隆位点，限制性内切酶

载体大小:6990 bp

3' 测序引物:Sing-Tet-U6-R: (4999–4967): 5'-GAAGC GGAAG AGCGC CCAAT ACGCA

AACCG CCT-3'

载体抗性:Ampicillin (氨苄青霉素)

筛选标记:Neomycin (新霉素)