Size | List Price |
Promotion Price | Stock |
Purchase |
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20 reactions | 1200.00 | 1200.00 | 1 |
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图片描述: | ||||
20 reactions |
质粒简介:
This is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold (activation by phosphorylation mutants of beta-catenin). The appropriate control plasmid is clone M51, Super8XFOPflash, which has mutant TCF/LEF binding sites.
This construct was made by Ajamete Kaykas in the Moon lab. The backbone is the pTA-Luc vector of Clontech, which provides a minimal TA viral promoter driving expression of the firefly luciferase gene (see company publications for details). 7 TCF/LEF binding sites were cloned into the Mlu1 site of this vector (7 copies of: AGATCAAAGGgggta, with TCF/LEF binding site in CAP letters, and a spacer in lower case, separating each copy of the TCF/LEF site).
Note: This plasmid was published as M50 Super 8x TOPFlash, but the plasmid actually contains 7 TCF/LEF sites.
产品参数:
质粒名称:M50 Super8x TOPFlash质粒
出品公司:Addgene
质粒类型:信号通路报告载体
载体大小:4985 bp
载体抗性:Ampicillin
报告基因:Luc
Product Name | Thumbnail |
---|---|
pGAS-TA-Luc | |
pSTAT3-TA-Luc | |
pISRE-TA-Luc | |
pTA-Luc | |
pIκB-EGFP | |
pNFAT-TA-Luc | |
pCaspase3-sensor | |
pAP1(PMA)-TA-Luc | |
pCRE-Luc | |
pGRE-Luc | |
pHSE-Luc | |
pE2F-TA-Luc | |
p53-Luc | |
pAP-1-Luc | |
pNF-κB-Luc |
For more information, please download and refer to the product manual。