pGL4.29[luc2P/CRE/Hygro] 载体

质粒类型:    启动子报告载体

克隆方法:    多克隆位点，限制性内切酶

载体大小:    6084 bp (查看载体序列)

载体抗性:    Ampicillin (氨苄青霉素)

筛选标记:    Hygromycin (潮霉素)

出品公司：Promega

备注:    Vector with a minimal promoter and a cAMP response element for studying cell signaling using destabilized luciferase.

载体描述

The pGL4.29[luc2P/CRE/Hygro] Vector(a–f) contains a cAMP response element (CRE) that drives the transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The luc2P gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly than the protein encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance.