pGL4.26[luc2/minP/Hygro] 载体

质粒类型:    启动子报告载体

克隆方法:    多克隆位点，限制性内切酶

载体大小:    5882 bp (查看载体序列)

载体抗性:    Ampicillin (氨苄青霉素)

筛选标记:    Hygromycin (潮霉素)

出品公司：Promega

备注:    Vector with a minimal promoter for measuring the activity of transcriptional response elements using a luciferase assay.

载体描述

The pGL4.26[luc2/minP/Hygro] Vector(a–e) encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The vector contains a multiple cloning region for insertion of a response element of interest upstream of a minimal promoter and the luc2 gene. luc2 is a synthetically-derived luciferase sequence with humanized codon optimization. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance.