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pAC149-pCR8-dCas9VP160 
   Cat.No QYV0305    
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Documentation and quality management
COA Datasheet
Basic product information:

BACKBONE

Vector backbone

pCR8/GW/TOPO

Backbone size w/o insert (bp)2799

Total vector size (bp)7442

Vector type

CRISPR ; Gateway Donor

GENE/INSERT

Gene/Insert namedCas9(D10A;H840A) fusion with VP160 activation domain
Alt namedCas9VP160
SpeciesH. sapiens (human), Synthetic
Insert Size (bp)4643
MutationD10A;H840A nuclease-deficient
Tags / Fusion Proteins
HA Tag (N terminal on insert)
VP160 (C terminal on insert)

GROWTH IN BACTERIA

Bacterial Resistance(s)

Spectinomycin

Growth Temperature

37°C

Growth Strain(s)

DH5alpha

Copy number

Unknown

CLONING INFORMATION

Cloning method:Restriction Enzyme

5′ cloning site:EcoRI (not destroyed)

3′ cloning site:EcoRI (not destroyed)

5′ sequencing primer:M13F

3′ sequencing primer:M13R

 
Other technical information

For more information, please download and refer to the product manual。

Please send your questions to us,thank you for your support!