pGL3-Control Vector

This vector can be obtained from Promega Corporation, Madison, WI. Call 
one of the following numbers for order or technical information.

Order or Technical     1-800-356-9526
Outside U.S.             608-274-4330

pGL3-Control Vector sequence reference points:

Base pairs                                    5256
Multiple cloning region                       1-41
Promoter                                      48-250
Luciferase gene (luc+)                        280-1932
GLprimer2 binding site                        281-303
SV40 late poly (A) signal                     1964-2185
Enhancer                                      2205-2441
RVprimer4 binding site                        2518-2499
ColE1-derived plasmid replication origin      2756
Beta-lactamase gene (Ampr)                    3518-4378
F1 origin                                     4510-4965
Synthetic (upstream) poly (A) signal          5096-5249
RVprimer3 binding site                        5198-5217

..
    1  GGTACCGAGC TCTTACGCGT GCTAGCCCGG GCTCGAGATC TGCGATCTGC

   51  ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG

  101  CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATC GCTGACTAAT

  151  TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC

  201  AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT

  251  GGCATTCCGG TACTGTTGGT AAAGCCACCA TGGAAGACGC CAAAAACATA

  301  AAGAAAGGCC CGGCGCCATT CTATCCGCTG GAAGATGGAA CCGCTGGAGA

  351  GCAACTGCAT AAGGCTATGA AGAGATACGC CCTGGTTCCT GGAACAATTG

  401  CTTTTACAGA TGCACATATC GAGGTGGACA TCACTTACGC TGAGTACTTC

  451  GAAATGTCCG TTCGGTTGGC AGAAGCTATG AAACGATATG GGCTGAATAC
    
  501  AAATCACAGA ATCGTCGTAT GCAGTGAAAA CTCTCTTCAA TTCTTTATGC

  551  CGGTGTTGGG CGCGTTATTT ATCGGAGTTG CAGTTGCGCC CGCGAACGAC

  601  ATTTATAATG AACGTGAATT GCTCAACAGT ATGGGCATTT CGCAGCCTAC

  651  CGTGGTGTTC GTTTCCAAAA AGGGGTTGCA AAAAATTTTG AACGTGCAAA

  701  AAAAGCTCCC AATCATCCAA AAAATTATTA TCATGGATTC TAAAACGGAT

  751  TACCAGGGAT TTCAGTCGAT GTACACGTTC GTCACATCTC ATCTACCTCC

  801  CGGTTTTAAT GAATACGATT TTGTGCCAGA GTCCTTCGAT AGGGACAAGA

  851  CAATTGCACT GATCATGAAC TCCTCTGGAT CTACTGGTCT GCCTAAAGGT

  901  GTCGCTCTGC CTCATAGAAC TGCCTGCGTG AGATTCTCGC ATGCCAGAGA

  951  TCCTATTTTT GGCAATCAAA TCATTCCGGA TACTGCGATT TTAAGTGTTG

 1001  TTCCATTCCA TCACGGTTTT GGAATGTTTA CTACACTCGG ATATTTGATA

 1051  TGTGGATTTC GAGTCGTCTT AATGTATAGA TTTGAAGAAG AGCTGTTTCT

 1101  GAGGAGCCTT CAGGATTACA AGATTCAAAG TGCGCTGCTG GTGCCAACCC

 1151  TATTCTCCTT CTTCGCCAAA AGCACTCTGA TTGACAAATA CGATTTATCT

 1201  AATTTACACG AAATTGCTTC TGGTGGCGCT CCCCTCTCTA AGGAAGTCGG

 1251  GGAAGCGGTT GCCAAGAGGT TCCATCTGCC AGGTATCAGG CAAGGATATG

 1301  GGCTCACTGA GACTACATCA GCTATTCTGA TTACACCCGA GGGGGATGAT

 1351  AAACCGGGCG CGGTCGGTAA AGTTGTTCCA TTTTTTGAAG CGAAGGTTGT

 1401  GGATCTGGAT ACCGGGAAAA CGCTGGGCGT TAATCAAAGA GGCGAACTGT

 1451  GTGTGAGAGG TCCTATGATT ATGTCCGGTT ATGTAAACAA TCCGGAAGCG

 1501  ACCAACGCCT TGATTGACAA GGATGGATGG CTACATTCTG GAGACATAGC

 1551  TTACTGGGAC GAAGACGAAC ACTTCTTCAT CGTTGACCGC CTGAAGTCTC

 1601  TGATTAAGTA CAAAGGCTAT CAGGTGGCTC CCGCTGAATT GGAATCCATC

 1651  TTGCTCCAAC ACCCCAACAT CTTCGACGCA GGTGTCGCAG GTCTTCCCGA

 1701  CGATGACGCC GGTGAACTTC CCGCCGCCGT TGTTGTTTTG GAGCACGGAA

 1751  AGACGATGAC GGAAAAAGAG ATCGTGGATT ACGTCGCCAG TCAAGTAACA

 1801  ACCGCGAAAA AGTTGCGCGG AGGAGTTGTG TTTGTGGACG AAGTACCGAA

 1851  AGGTCTTACC GGAAAACTCG ACGCAAGAAA AATCAGAGAG ATCCTCATAA

 1901  AGGCCAAGAA GGGCGGAAAG ATCGCCGTGT AATTCTAGAG TCGGGGCGGC

 1951  CGGCCGCTTC GAGCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC

 2001  CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG

 2051  CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC

 2101  AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT

 2151  TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTAAAATC GATAAGGATC

 2201  TGAACGATGG AGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA

 2251  TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG

 2301  CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT

 2351  TGCTGACTAA TTGAGATGCA TGCTTTGCAT    ACTTCTGCCT GCTGGGGAGC

 2401  CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG CGGATCCGTC

 2451  GACCGATGCC CTTGAGAGCC TTCAACCCAG TCAGCTCCTT CCGGTGGGCG

 2501  CGGGGCATGA CTATCGTCGC CGCACTTATG ACTGTCTTCT TTATCATGCA

 2551  ACTCGTAGGA CAGGTGCCGG CAGCGCTCTT CCGCTTCCTC GCTCACTGAC

 2601  TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA

 2651  GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA

 2701  TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG

 2751  CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG

 2801  ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG

 2851  CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG

 2901  CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC

 2951  TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA

 3001  AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA

 3051  TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC

 3101  ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG

 3151  GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA

 3201  ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG

 3251  AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT

 3301  TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA

 3351  GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC

 3401  ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA

 3451  TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG

 3501  TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC

 3551  AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT

 3601  AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG

 3651  ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA

 3701  GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT

 3751  CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA

 3801  GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC

 3851  ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA

 3901  GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC

 3951  GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT

 4001  GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT

 4051  GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT

 4101  ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC

 4151  GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG

 4201  GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA

 4251  CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT

 4301  TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA

 4351  GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT

 4401  TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG

 4451  TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG

 4501  TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG

 4551  GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC

 4601  TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC

 4651  AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG

 4701  CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC

 4751  ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT

 4801  TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG

 4851  GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT

 4901  AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT

 4951  TAACGCTTAC AATTTGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA

 5001  GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCCCA AGCTACCATG

 5051  ATAAGTAAGT AATATTAAGG TACGGGAGGT ACTTGGAGCG GCCGCAATAA

 5101  AATATCTTTA TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGAATCGA

 5151  TAGTACTAAC ATACGCTCTC CATCAAAACA AAACGAAACA AAACAAACTA

 5201  GCAAAATAGG CTGTCCCCAG TGCAAGTGCA GGTGCCAGAA CATTTCTCTA

 5251  TCGATA

Sequence revised 16-Apr-02.