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  •  Western blot analysis of VEC using p-Rpb1 (S1619) antibody. Antibody was diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
  •  Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using POLR2A (Phospho-Ser1619) Antibody
  •  Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.
  •  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is blocked with the phospho peptide.
  •  Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using POLR2A (Phospho-Ser1619) Antibody. The lane on the right is blocked with the phospho peptide.
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Rpb1 (phospho Ser1619) Polyclonal Antibody 
   Cat.No QYA08745    
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100ug/100ul 2250.00 1350.00
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100ug/100ul
Documentation and quality management
COA Datasheet
Basic product information:

Catalog No.

QYA08745

Size.

100µg/100µl

Gene Name.

POLR2A

Protein Name.

DNA-directed RNA polymerase II subunit RPB1

Source.

Rabbit

Immunogen.

Synthesized phospho-peptide around the phosphorylation site of human Rpb1 (phospho Ser1619)

Purification.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity.

Phospho-Rpb1 (S1619) Polyclonal Antibody detects endogenous levels of Rpb1 protein only when phosphorylated at S1619.

Formulation.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Concentration.

1 mg/ml

Storage / Stability.

-20°C/1 year

Human Gene ID.

5430

Human Swiss-Prot No.

P24928

Mouse Swiss-Prot No

P08775

Reactivity.

Human, Mouse, Rat, Monkey

Applications.

WB,IHC-p,IF,ELISA

Predicted band (Da)

217206

Observed band (KDa)

250

Dilution.

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Other Name

POLR2A; POLR2; DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1

Other technical information

For more information, please download and refer to the product manual。

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