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  •  Immunofluorescence analysis of human-breast-cancer tissue. 1,p53 (Di Methyl Lys370) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  •  Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,p53 (Di Methyl Lys370) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  •  Western Blot analysis of MCF7, K562 cells using Di-Methyl-p53 (K370) Polyclonal Antibody.. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
  •  Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100
  •  Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
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p53 (Di Methyl Lys370) Polyclonal Antibody 
   Cat.No QYA07402    
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100ug/100ul 2250.00 1350.00
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100ug/100ul
Documentation and quality management
COA Datasheet
Basic product information:

Catalog No.

QYA07402

Size.

100ug

Gene Name.

TP53

Protein Name.

Cellular tumor antigen p53

Source.

Rabbit

Immunogen.

Synthesized peptide derived from human p53 around the di-methylation site of K370.

Purification.

The antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogen.

Specificity.

Di-Methyl-p53 (K370) Polyclonal Antibody detects endogenous levels of p53 protein only when di-methylated at K370.

Formulation.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration.

1 mg/ml

Storage / Stability.

-20°C/1 year

Human Gene ID.

7157

Human Swiss-Prot No.

P04637

Mouse Gene ID

 

Mouse Swiss-Prot No.

P02340

Rat Gene ID

 

Rat Swiss-Prot No.

 

Reactivity.

Human

Applications.

IF,WB,IHC-p,ELISA

Predicted band (Da)

 

Observed band (KDa)

53

Dilution.

IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.

Other Name

 

Other technical information

For more information, please download and refer to the product manual。

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