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  •  Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,NFκB-p65 (Acetyl Lys310) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  •  Immunofluorescence analysis of Human-appendix tissue. 1,NFκB-p65 (Acetyl Lys310) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  •  Western Blot analysis of various cells using Acetyl-NFκB-p65 (K310) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
  •  Western Blot analysis of Hela cells using Acetyl-NFκB-p65 (K310) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
  •  The picture was kindly provided by our customer,antibody was diluted at 1:500
  •  Immunofluorescence analysis of HeLa cells, using NF-kappaB p65 (Acetyl-Lys310) Antibody. The picture on the right is blocked with the synthesized peptide.
  •  Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NF-kappaB p65 (Acetyl-Lys310) Antibody. The picture on the right is blocked with the synthesized peptide.
  •  Western blot analysis of lysates from HeLa cells, treated with TSA 40nM 24h, using NF-kappaB p65 (Acetyl-Lys310) Antibody. The lane on the right is blocked with the synthesized peptide.
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NFκB-p65 (Acetyl Lys310) Polyclonal Antibody 
   Cat.No QYA06563    
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100ug/100ul 2250.00 1350.00
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100ug/100ul
Documentation and quality management
COA Datasheet
Basic product information:

Catalog No.

QYA06563

Size.

100ug

Gene Name.

RELA

Protein Name.

Transcription factor p65

Source.

Rabbit

Immunogen.

The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the acetylated site of Lys310. AA range:275-324

Purification.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity.

Acetyl-NFκB-p65 (K310) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when acetylated at K310.

Formulation.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration.

1 mg/ml

Storage / Stability.

-20°C/1 year

Human Gene ID.

5970

Human Swiss-Prot No.

Q04206

Mouse Gene ID.

19697

Mouse Swiss-Prot No.

Q04207

Rat Gene ID.

 

Rat Swiss-Prot No.

 

Reactivity.

Human,Mouse

Applications.

WB,IHC-p,IF,ELISA

Predicted band (Da)

60219

Observed band (KDa)

65

Dilution.

WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300

Other name.

RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Other technical information

For more information, please download and refer to the product manual。

Please send your questions to us,thank you for your support!